For diploid organisms, such targeted mutations may happen in one or both homologous chromosomes. Mutations induced by CRISPR/Cas9 usually occur around the cleavage sites at three bases upstream of the protospacer-adjacent motif (PAM), producing insertion and deletion of nucleotides. Rapid Decoding of Sequence-Specific NucleaseInduced Heterozygous and Biallelic Mutations by Direct Sequencing of PCR Products Dear Editor, The recent development of sequence-specific nuclease systems, i.e., TALENs and CRISPR/Cas9, has made genomic targeting easier in many organisms including plants (Li et al., 2012 Cong et al., 2013 Joung and Sander, 2013 Li, et al., 2013 Shan et al., 2013 Liang et al., 2014 Zhang et al., 2014).
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